Editing the Genome Without Double-Stranded DNA Breaks
نویسندگان
چکیده
منابع مشابه
Editing the Genome Without Double-Stranded DNA Breaks.
Genome editing methods have commonly relied on the initial introduction of double-stranded DNA breaks (DSBs), resulting in stochastic insertions, deletions, and translocations at the target genomic locus. To achieve gene correction, these methods typically require the introduction of exogenous DNA repair templates and low-efficiency homologous recombination processes. In this review, we describ...
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Genome editing using transcription-activator like effector nucleases or RNA guided nucleases allows one to precisely engineer desired changes within a given target sequence. The genome editing reagents introduce double stranded breaks (DSBs) at the target site which can then undergo DNA repair by non-homologous end joining (NHEJ) or homology directed recombination (HDR) when a template DNA mole...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2017
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.7b00710